SH2 Superbinders, aka SH2S, are variants of the Src homology
2 domain that have >100x greater affinity for Tyr phosphorylated proteins
and pTyr peptides than any wild-type SH2 domain
Right picture: a Western blot image showing that the SH2S Magic agarose beads
from Precision Proteomics captured essentially all pTyr proteins from the Jurkat T cells
When used as an affinity reagent for pTyr peptide enrichment, the SH2S Magic beads
Outperformed anti-pTyr antibodies in parallel AP-MS/MS analysis of the Tyr
phosphoproteome.
Right picture: a summary of Tyr phosphorylated proteins identified from 0.5 mg Hela cell lysate
by AP-MS/MS using either the SH2S Magic beads or anti-pTyr antibody conjugated agarose beads.
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